Shorter storage durations of donor human milk may reduce gastrointestinal complications in premature infants, including necrotizing enterocolitis, according to new research. Minimizing milk storage time may help preserve protective properties that are critical for preterm gut health.
The researchers believe their findings may necessitate updated WHO and CDC guidance, as both currently advise that frozen milk can be stored safely for up to a year for all babies.
“Our results showed that babies weren’t as protected with the use of donor milk that was stored for a longer time as with donor milk that was stored for shorter periods of time,” says lead study author, neonatalist Katherine Chetta, M.D., from the US Medical University of South Carolina (MUSC).
Chetta’s research program focuses on the science of breast milk, nutrition, and intestinal rehabilitation.
Essential lifeline
Donor human milk is a vital substance for almost all preterm babies when their own mother’s milk is unavailable, in short supply, or unsafe. Feeding with breast milk reduces complications associated with formula, while supporting immune function and digestion.
Human breast milk is particularly essential for infants with specific medical conditions, such as low blood sugar, congenital heart disease, and immunologic deficiencies.
One of the most significant benefits of donor milk is reducing the risk of necrotizing enterocolitis (NEC). This severe inflammatory disease affects mainly preemies and can lead to bowel tissue dying. It requires medication and often surgery and can lead to lifelong complications.
Chetta collaborates closely with the nutrition management center, or “milk room,” at the Medical University of South Carolina, where the donor milk is stored.
Based on previous studies and detailed analysis of the milk, Chetta and her team knew that longer storage times mean a breakdown in the action and efficacy of specific milk components.
While medical researchers have not yet identified the sole cause of NEC, they have posited that several connected factors can injure the premature gut, allowing bacteria in and creating inflammation.
This led to the researchers to ask whether aging milk is less protective against the development of NEC than fresher milk.
Dangers of longer milk storage
At the MUSC Shawn Jenkins Children’s Hospital, Chetta has long dealt with the devastating effects of NEC in her patients. “It’s one of those key benchmark diseases that all NICUs are constantly trying to decrease,” she notes.
Chetta reviewed the medical records of 262 very-low-birth-weight, less than 3.3 lb, infants who were provided donor milk in the NICU at the MUSC Shawn Jenkins Children’s Hospital between February 2022 and January 2024.
Among those premature babies, 226 remained free of disease while 36 developed NEC or another gut disease called spontaneous perforation, which also causes tremendous damage to intestinal tissue.
Chetta’s findings revealed that every extra day of storage increased the risk of NEC in very-low-birth-weight preemies by 3.7%. Her team notes that the “sweet spot” for frozen milk storage was approximately 240 days for the tiniest infants.
Laboratory results revealed that specific milk components degrade even while in frozen storage over time.
“When preemies are on the edge of viability, and extremely low birth weight, that’s when they seem to be the most susceptible to the effects of storage and can be most protected by having fresher milk,” says Chetta. “[For preemies] fresh milk is the best.”
Need for reevaluating WHO guidelines
Based on these findings, Chetta and her team believe that world health guidelines need to be reevaluated, especially regarding critically ill babies. The discovery has already influenced clinical practice in Chetta’s NICU.
“We’re so convinced that we need a little bit fresher that we’re already doing it just to be on the safer side,” says Chetta. “That’s how much we believe in it.”
The researchers caution that this doesn’t mean discarding or wasting milk that is not relatively as fresh. Instead, they urge the use of fresh milk more quickly and the optimization of freezing and storage procedures so that the sickest and youngest babies can access the freshest milk.
Spreading knowledge
Chetta has worked closely with the Mother’s Milk Bank of South Carolina and others involved in handling human milk.
“Other NICUs could talk to their own milk banks about bringing this milk in a little bit quicker from donor depot sites, as we did,” she explains. “We didn’t have to change much. We were just refocused on trying to get it under that threshold of 240 days.”
The researchers have high hopes for the impact of their study in the near future. “I think improving outcomes can be expanded very rapidly once people start verifying some of the findings we have,” says Chetta.
“From a policy standpoint, it could be a quick change. I hope using fresher milk will make a difference in the lives of the youngest babies. I think it will.”
The study from the is published in the Journal of Parenteral and Enteral Nutrition, reveals that
In other research on chronological breast milk storage, scientists discovered this year that certain breast milk hormones — melatonin and cortisol — and proteins involved in this process vary in concentration over the course of a day, which may influence babies’ rest.
