Author: admin

In addition, there was strong evidence that many of the brands did not use fit-for-purpose testing, according to Katie Banaszewski, senior director of quality at NOW.The Illinois-based company purchased two samples of 22 different brands on Amazon.com in August 2025. One bottle of each sample was tested in NOW’s in-house lab in Illinois, and the other was tested by ISO 17025-accredited Alkemist Labs in California.Hypericin, a key marker compound for St. John’ wort (Hypericum perforatum), was quantified by High-Performance Liquid Chromatography (HPLC) using a validated reverse-phase method after irradiation with white light to ensure specificity. Hypericin content was expressed as…

Cell linesKP LUAD cell lines were obtained from the laboratory of T. Jacks. KP, Fsp1KO cell lines were generated by transient transfection of PX458 (Addgene #48138) expressing an sgRNA targeting Fsp1. Single GFP-positive clones were selected and FSP1 loss was validated by western blot. The 16645 cell line was developed from KrasG12D Stk11−/− GEMM as previously described52. KPC7 cells were obtained from the laboratory of D. Simeone. All cell lines were maintained in DMEM or RPMI 160 (Corning) supplemented with 10% FBS (Sigma Aldrich) and gentamicin (Invitrogen) and were tested for mycoplasma regularly (PlasmoTest, InvivoGen). All mouse cell lines were…

Researchers have pinpointed specific metabolic pathways through which exercise counterbalances the negative behavioral effects of consuming a Western-style cafeteria diet. In a new study, voluntary running exercise was effective at mitigating depression-like behaviors induced by high-fat, high-sugar diets that impact circulating hormones and gut-derived metabolites.Adult male rats in the study were fed either “standard chow” or a “rotating cafeteria diet” that consisted of various high-fat and high-sugar foods for seven and a half weeks. Half of each dietary population had access to running wheels. This experimental design isolated the independent and combined effects of diet quality and physical activity on…

☕❓ What Are Black Coffee Benefits and Side Effects? Black coffee is one of the simplest — and most misunderstood — drinks in the world. Packed with caffeine and antioxidants, it can sharpen your mind, support metabolism, and even protect certain organs. But how much is too much, and when does it turn harmful? Here’s what science — and your body — really say about that daily cup. ☀️ The World’s Favorite Morning Habit — With a Dark Side? For billions of people, that first sip of steaming black coffee is sacred. It’s the ritual that draws a line between…

Human neural organoid research is booming, but the field lacks the proper legal and ethical guidelines to regulate it. Neuroscientists and bioethicists are hoping to change this.Image credit:©iStock, jroballoIn a 2022 study, scientists transplanted a cluster of human neurons into developing rat brains and discovered that these cells integrated and matured alongside rat neurons.1 The researchers could even activate the human cells by touching the rats—which sounds like a scene from a science fiction movie.“You tickle the rat whiskers, and the human cells fire,” said Henry (Hank) Greely, a bioethicist at Stanford University. “There’s a lot of weird stuff here.”Human…

As a physician, one of the most common questions I get from patients trying to improve their health is, “What’s the one simple switch I can make?” Often, their focus lands on bread. We’re a nation that loves our sandwiches, toast, and sides, but we’re also wary of “empty carbs.” This leads many to the dense, dark loaves in the bakery aisle, asking: Is rye bread healthy? The short answer is a resounding yes, for the right person. When made from 100% whole grains, rye bread is a nutritional powerhouse. It’s packed with fiber, linked to better blood sugar control,…

Leaky gut occurs when your gut barrier becomes compromised, allowing substances to “leak” through.Diarrhea, bloating, brain fog or widespread pain or inflammation are common symptoms of leaky gut.Experts suggest avoiding triggers, eating plenty of fiber and polyphenols and more to manage leaky gut. Gut conditions are more common than you might realize; 60 to 70 million people in the U.S. are impacted by some form of digestive issue. One disorder you may have heard of lately is leaky gut syndrome. “Leaky gut has become a bit of a buzz phrase, but it is not a medically diagnosable condition”, explains Treta…

Carlos Dora, visiting professor of public policies1, Eugenio Scannavino Netto, founding director2, Andre Guimaraes, executive director31Federal University Rio Grande do Sul, Brazil 2Projeto Saude e Alegria, Amazon, Brazil 3Instituto de Pesquisa Ambiental da Amazonia (IPAM), BrazilCorrespondence to: C Dora carlos.f.dora{at}gmail.comProtecting forests and inhabitants will pay health dividendsThe Amazon rainforest is the world’s largest tropical forest and one of its most vital ecosystems for human health and planetary stability. It stores the equivalent of 15-20 years of global carbon emissions and regulates rainfall patterns critical to food security across Latin America and beyond.1 The forest’s loss could trigger catastrophic warming—up to…

Developing human brain tissue containing rosette structures (pink and blue) surrounded by neurons (green).Credit: Nano et al./Nature Neurosci.; Jose Soto/Bhaduri Lab/UCLAScientists have created the most detailed maps yet of how our brains differentiate from stem cells during embryonic development and early life. In a collection of five papers published in Nature on 5 November, they tracked hundreds of thousands of early brain cells in the cortexes of humans and mice, and captured with unprecedented precision the molecular events that give rise to mixture of neurons and supporting cells.“It’s really the initial first draft of any ‘cell atlases’ for the developing…

A data set nearly doubles the known length of roads used during the Roman Empire. Credit: Itiner-e, Artas Media, MINERVAA high-resolution digital map allows people to plan their routes along the ancient roads of the Roman Empire. Combining historical records with modern mapping techniques, researchers mapped hundreds of thousands of kilometres of roads. The findings nearly double the known length of Roman roads.The data set was published in Scientific Data on 6 November alongside an online platform called Itiner-e, which study co-author Tom Brughmans calls a “Google Maps for Roman roads”1.“It’s a growing resource for a community to keep on…